The effectiveness of a combination of probiotics and natural wheat and sesame based prebiotics in enhancing the growth performance of giant freshwater prawns (Macrobrachium rosenbergii)
DOI:
https://doi.org/10.30997/jmss.v12i1.19233Keywords:
Synbiotic, Giant Freshwater Prawns, Intestinal Histology, Natural Materials, GrowthAbstract
The main challenge in farming M. rosenbergii is its relatively slow growth rate, which directly affects productivity and profitability. This research evaluates the effectiveness of probiotic and prebiotic combinations based on wheat and sesame (synbiotics) on the growth performance of giant prawns. This research used a Completely Randomized Design (CRD) with four treatments: Control (P1), Probiotic (P2), Wheat-based Synbiotic (P3), and Sesame-based Synbiotic (P4), each replicated three times. Observed parameters included growth performance, feed conversion ratio (FCR), survival rate, and intestinal histology. Treatment P3 provided the best result with an average weight gain of 4.03 ± 1.85 g, a specific growth rate (SGR) of 0.61 ± 0.23% per day, a length gain of 1.82 ± 0.45 cm, and a specific growth rate of length (SLGR) of 0.27 ± 0.06% per day. The lowest Feed Conversion Ratio (FCR) was found in P3, at 2.66 ± 0.53 g, indicating the best feed efficiency and more uniform and longer intestinal microvilli. The survival rate in P3 reached 93.33 ± 11.55%. Treatment P3 improved growth, feed efficiency, and intestinal health in giant prawns. Additional studies are suggested to investigate the molecular mechanisms by which synbiotics affect digestive and immune-related genes.
References
Akter, M. N., Zahan, K., Zafar, M. A., Khatun, N., Rana, M. S., & Mursalin, M. I. (2021). Effects of Dietary Mannan Oligosaccharide on Growth Performance, Feed Utilization, Body Composition and Haematological Parameters in Asian Catfish (Clarias batrachus) Juveniles. Turkish Journal of Fisheries and Aquatic Sciences, 21(11), 559-567. http://doi.org/10.4194/1303-2712-v21_11_04
Al-Hafedh, Y. (2008). Effects of Dietary Protein Level on Growth, Feed Conversion, and Protein Efficiency Ratio of Freshwater Prawn, Macrobrachium rosenbergii, in Outdoor Concrete Tanks. Journal of Applied Aquaculture, 14(17), 51–60. https://doi.org/10.1300/J028v19n01_05
Arığ, N., Suzer, C., Gökvardar, A., Başaran, F., Çoban, D., Yıldırım, Ş., Kamacı, H.O., Fırat, K., & Saka, Ş. (2013). Effects of Probiotic (Bacillus sp.) Supplementation during Larval Development of Gilthead Sea Bream (Sparus aurata, L.). Turkish Journal of Fisheries and Aquatic Sciences, 13, 407 - 414. 10.4194/1303-2712-v13_3_03
Azhdari, S. M. H., Pournaki, Shirin, K., Tavabe, K. R., Hosseni, S. V., Javanmardi, D. B. S., Azhdari, A., & Frinsko, M. (2003). Effects of Bacillus subtilis and Lactobacillus plantarum Probiotics on the Litopenaeus vannamei Growth Performance, Hemolymph Factors, and Physicochemical Parameters. Aquaculture Reports, 33. https://doi.org/10.1016/j.aqrep.2023.101873
Butt, U. D., Lin, N., Akhter, N., Siddiqui, T., Li, S., & Wu, Bin. (2021). Overview of the Latest Developments in the Role of Probiotics, Prebiotics and Synbiotics in Shrimp Aquaculture. Fish & Shellfish Immunology, 114, 263–281. https://doi.org/10.1016/j.fsi.2021.05.003
Butucel, E., Balta, I., McCleery, D., Marcu, A., Stef, D., Pet, I., Callaway, T., Stef, L., & Corcionivoschi, N. (2023). The Prebiotic Effect of an Organic Acid Mixture on Faecalibacterium prausnitzii Metabolism and Its Anti-Pathogenic Role against Vibrio parahaemolyticus in Shrimp. Biology, 12, 1–13. https://doi.org/10.3390/biology12010057
Carlson, J. L., Erickson, J. M., Lloyd, B. B., & Slavin, J. L. (2018). Health Effects and Sources of Prebiotic Dietary Fiber. Current Developments in Nutrition, 2(3), 1-26 doi:10.1093/cdn/nzy005. https://doi.org/10.1093/cdn/nzy005
Chen, M., Chen, X., Tian, L., Liu, Y., & Niu, J. (2020). Beneficial Impacts on Growth, Intestinal Health, Immune Responses, and Ammonia Resistance of Pacific White Shrimp (Litopenaeus vannamei) Fed Dietary Synbiotic (Mannan Oligosaccharide and Bacillus licheniformis). Aquaculture Reports, 17, 1-11. https://doi.org/10.1016/j.aqrep.2020.100408
Cisneros, J., Fuentes-Valencia, M. A., Davizón, Y. A., Marquéz-Pacheco, H., Leyva-Morales, J. B., Valencia-Castañeda, G., Maldonado-Coyac, J. A.,Ontiveros-García, L. A., & Badilla-Medina, C. N. (2023). Prebiotics in Global and Mexican Fish Aquaculture: A Review. Animals, 13(3607), 1-2. https://doi.org/10.3390/ani13233607
Cruz, P. M., Iba´nez, A. L., Hermosillo, O. A. M. & Saad, H. C. R. (2012). Review Article: Use of Probiotics in Aquaculture. International Scholarly Research Network, 1-13. https://doi.org/10.5402/2012/916845
Davani-Davari, D., Negahdaripour, M., Karimzadeh, I., Seifan, M., Mohkam, M., Masoumi, S. J., Berenjian, A., & Ghasemi, Y. (2019). Prebiotics: Definition, Types, Sources, Mechanisms, and Clinical Applications. Foods, 8(3), 92. https://doi.org/10.3390/foods8030092
Dhingra, D., Michael, M., Rajput, H. & Patil, R. T. (2012). Dietary Fibre in Foods: A Review. Journal Food Sci Technol, 49(3), 255–266. https://doi.org/10.1007/s13197-011-0365-5
El-Saadony, M. T., Alagawany, M., Patra, A. K., Kar, I., Tiwari, R., Dawood, M. A. O., Dhama, K., & M. R. A, Hany. (2021). The Functionality of Probiotics in Aquaculture: An overview. Fish & Shellfish Immunology, 117, 36-52. https://doi.org/10.1016/j.fsi.2021.07.007
Escobedo-Fregoso, C., Quiroz-Guzmán, E., Mendoza-Carrion, G. & Peña-Rodríguez, A. (2021). Effect of Dietary Prebiotic Inulin and Probiotic Bacillus subtilis And Lactobacillus sp., on The Intestinal Microbiota of White Shrimp Litopenaeus vannamei. Biotecnica, 23(3), 50-57. https://doi.org/10.18633/biotecnia.v23i3.1440
FAO. (2022). FAO (Food and Agriculture Organization of the United Nations). Database on Introductions of Aquatic Species. https://www.fao.org/fishery/en/topic/14786/en.
FAO, (2022). FAO (Food and Agriculture Organization of the United Nations). The State of World Fisheries and Aquaculture 2022. Towards Blue Transformation. Rome: FAO. https://doi.org/10.4060/cc0461en.
Fahrudin, A. M., Subandiyono, Chilmawati, D. (2023). Pengaruh Protein dalam Pakan terhadap Efisiensi Pemanfaatan Pakan dan Pertumbuhan Juvenil Vaname (Litopenaeus vannamei). Jurnal Sains Akuakultur Tropis, 1, 114-126
Farook, M. A., Mohamed, H. S. M., Tariq, N., Shariq, K. M., & Ahmed, I. A. (2019). Giant Freshwater Prawn, Macrobrachium rosenbergii (De Man, 1879): A Review. IJRAR- International Journal of Research and Analytical Reviews, 6(1), 571–584.
Felgenhauer, B. (1992). Internal Anatomy of the Decapoda: An Overview. Microscopic Anatomy of Invertebrates, 10, 45-75.
Fidyandini, H. P., Elisianda, Y., & Kartini, N. (2020). Pelatihan Penggunaan Probiotik and Imunostimulan untuk Pencegahan and Pengobatan Penyakit Ikan Lele pada Kelompok Pembudidaya Ikan Ulam Adi Jaya Kabupaten Mesuji. Jurnal Sinergi, 1(8), 50-54. 10.23960/js.v1i1.8
Flint, H. J., Duncan, S. H., Scott, K. P. & Louis, P. (2015). Links Between Diet, Gut Microbiota Composition, and Gut Metabolism. Proceedings of the Nutrition Society, 74(1), 13–22. https://doi.org/10.1017/s0029665114001463
Gatlin, DM, Barrows, FT, Brown, P., Dabrowski, K., Gaylord, TG, Hardy, RW, Herman, E., Hu G., Krogdahl, A., Nelson, R., Overturi, K., Rust, M., Sealey W., Skonberg, D., Souza, E.J., Stone, D., Wilson, R., & Wurtele, E. (2007). Expanding the utilization of sustainable plant products in aquafeeds: a review. Aquaculture Research, 38(6), 551–579. doi:10.1111/j.1365-2109.2007.01704.x
Gibson, G. R., & Wang, X. (1994). Enrichment of Bifidobacteria from Human Gut Contents by Oligofructose Using Continuous Culture. FEMS Microbiology Letters, 118(1-2), 121–127. 10.1111/j.1574-6968.1994.tb06813.x
Hooper, C., Debnath, P. P., Stentiford, G. D. Bateman, K. S., Salin, K. R., & Bass, David. (2023). Diseases of the giant river prawn Macrobrachium rosenbergii: A review for a growing industry. Reviews in Aquaculture, 15, 738–758. https://doi.org/10.1111/raq.12754
Hoseinifar, S. H., Sun, Y.-Z., Wang, A. & Zhou, Z., (2018). Probiotics as Means of Diseases Control in Aquaculture, a Review of Current Knowledge and Future Perspective. Frontiers in Microbiology, 9, 1-18. https://doi.org/10.3389/fmicb.2018.02429
Isolauri, E., Sütas, Y., Kankaanpää, P., Arvilommi, H., & Salminen, S. (2001). Probiotics: Effects on Immunity. The American Journal of Clinical Nutrition, 73(2), 444s-450s. https://doi.org/10.1093/ajcn/73.2.444s
Kaur, A. P., Bhardwaj, S., Dhanjal, D. S., Nepovimova, E., Cruz-Martins, N., Kuča, K., Chopra, C., Singh, R., Kumar, H., Șen, F., Kumar, V., Verma, R., & Kumar, D. (2021). Plant Prebiotics and Their Role in the Amelioration of Diseases. Biomolecules, 11(3), 1–25. https://doi.org/10.3390/biom11030440
Khanjani, M. H., Mozanzadeh, M. T., Gisbert, E. & Hoseinifar, S. H. (2024). Probiotics, Prebiotics, and Synbiotics in Shrimp Aquaculture: Their Effects on Growth Performance, Immune Responses, and Gut Microbiome. Aquaculture Reports, 38. https://doi.org/10.1016/j.aqrep.2024.102362
Klahan, R., Deevong, P., Wiboonsirikul, J. & Yuangsoi, B. (2023). Growth Performance, Feed Utilisation, Endogenous Digestive Enzymes, Intestinal Morphology, and Antimicrobial Effect of Pacific White Shrimp (Litopenaeus vannamei) Fed with Feed Supplemented with Pineapple Waste Crude Extract as a Functional Feed Additive. Aquaculture Nutrition, 1-13. https://doi.org/10.1155/2023/1160015
Mamun, M. A. A., Nasren, S., Rathore, S. S., Sidiq, M. J., Dharmakar, P., & Anjusha, K. V. (2019). Assessment of Probiotics in Aquaculture: Functional Changes and Impact on Fish Gut. Microbiology Research Journal International, 29(1), 1-10. https://doi.org/10.9734/mrji/2019/v29i130156
Mao, X.-j., & Lu, K.-l. (2023). Fish Nutrition and Physiology. Fishes, 8(8), 401. https://doi.org/10.3390/fishes8080401
Merrifield, D. L., Bradley G., Baker, R. T. M., & Davies, S. J.(2010). Effects on Growth Performance, Feed Utilization, Intestinal Microbiota and Related Health Criteria Postantibiotic Treatment, 16(6), 496-506. doi: 10.1111/j.1365-2095.2009.00688.
Miran, M., Salami, M., Yarmand, M. S., Ferreira-Lazarte, A., Ariaeenejad, S., Montilla, A., & Moreno, F. J. (2024). Arabinoxylo-Oligosaccharides Production from Unexploited Agro-Industrial Sesame (Sesamum indicum L.) Hulls Waste. Carbohydrate Polymers, 342. https://doi.org/10.1016/j.carbpol.2024.122399
Mohan, K., Muralisankarb, T., Uthayakumara, V., Chandirasekara, R., & Rajan, D. K. (2019). Dietary Ganoderma Lucidum Polysaccharides to Enhance The Growth, Immune Response and Disease Resistance of Freshwater Prawn Macrobrachium rosenbergii. Aquaculture Reports, 14, 1-9. https://ui.adsabs.harvard.edu/link_gateway/2019AqRep..1400203M/doi:10.1016/j.aqrep.2019.100203
Mohapatra, S., Chakraborty, T., Kumar, V., De, B. G., & Mohanta, K. N. (2012). Aquaculture and Stress Management: A Review of Probiotic Intervention. Journal of Animal Physiology and Animal Nutrition, 14, 1–26. https://doi.org/10.1111/j.1439-0396.2012.01301.x
Moshfegh, A. J., Friday, J. E., Goldman, J. P. & Ahuja, J. K. C. (1999). Presence of inulin and oligofructose in the diets of Americans. The Journal of Nutrition, 129(7), 1407–1411. https://doi.org/10.1093/jn/129.7.1407s
Müller, M., Hermes, G. D. A., Canfora, E. E. Holst, J. J., Zoetendal, E. G., Smidt, Hauke., Troost, Freddy., Schaap, F. G., Damink, S. O., Jocken, J. W. E., Lenaerts, K., Masclee, Ad A. M., & Blaak, E. E. (2020). Effect of Wheat Bran Derived Prebiotic Supplementation on Gastrointestinal Transit, Gut Microbiota, and Metabolic Health: A Randomized Controlled Trial in Healthy Adults With a Slow Gut Transit. Gut Microbes, 12(1), 1-14. https://doi.org/10.1080/19490976.2019.1704141
New, M. B. (2005). Freshwater prawn farming: Global status and trends. Aquaculture Research, 36(3), 210-230. doi: 10.1111/j.1365-2109.2005.01237
Nuryati, S., Yanti , M., Rahman & Wahyuwarandi, S. (2023). Kidney and Liver Histopathology of Sea Bass Lates calcarifer yang Diinfeksi Bakteri yang Berasosiasi dengan Black Body Syndrome. Jurnal Akuakultur Indonesia, 22(2), 170-178. http://dx.doi.org/10.19027/jai.22.2.170-178
Pepi, M. & Focardi, S. (2021). Antibiotic-Resistant Bacteria in Aquaculture and Climate Change: A Challenge for Health in the Mediterranean Area. International Journal Environmental Research Public Health, 18(11), 5723. https://doi.org/10.3390/ijerph18115723.
Prabu, E., Felix, S., Felix, N., Ahilan, B., Ruby, P. (2017). An overview of the significance of fish nutrition in the aquaculture industry. International Journal of Fisheries and Aquatic Studies, 5(6), 349–355. http://www.fisheriesjournal.com/archives/2017/vol5issue6/PartE/5-6-30-285.pdf
Pritchard, J. R., Lawrence, G. J., Larroque, O., Li, Z., Laidlaw, H. K. C., Matthew K., & Rahmana, S. (2011). A Survey of Β-Glucan and Arabinoxylan Content in Wheat. Journal of the Science of Food and Agriculture, 91, 1298–1303. https://doi.org/10.1002/jsfa.4316
Prosky, L., Asp, N.-G., Scheweizer, T. F., DeVries, J. W., & Furda, I. (1988). Determination of Insoluble, Soluble, and Total Dietary Fiber in Foods and Food Products: Interlaboratory Study. Journal of the Association of Official Analytical Chemists, 71(5), 1017-1023.
Ringø, E., Løvmo, L., Kristiansen, M., Bakken, Y., Salinas, I., Myklebust, R., Olsen, R. E., Mayhew, T. M. (2010). Lactic Acid Bacteria vs. Pathogens in the Gastrointestinal Tract of Fish: a review. Aquaculture Research, 41(4), 451–467. doi:10.1111/j.1365-2109.2009.02339.x
Srinivasan, V., Bhavan, P Saravana., Rajkumar, G., Satgurunathan, T., & Muralisankar, T. (2016). Effects of Dietary Iron Oxide Nanoparticles on The Growth Performance, Biochemical Constituents and Physiological Stress Responses of The Giant Freshwater Prawn Macrobrachium rosenbergii Post-larvae. International Journal of Fisheries and Aquatic Studies, 4(2), 170-182.
Sukenda, Praseto, R. & Darni, W. (2015). Efektivitas Sinbiotik Dengan Dosis Berbeda Pada Pemeliharaan Udang Vaname Di Tambak. Jurnal Akuakultur Indonesia, 14(1), 1-8.
Sutriana, A., Akter, M., Hashim, R. & Nor, S. (2025). Effectiveness of Galactooligosaccharide and Combination of Yeast + Β-Glucan in Soybean Meal Diets on Innate Immune Response and Disease Resistance Against Aeromonas hydrophila in Striped Catfish (Pangasianodon hypophthalmus). Turkish Journal of Fisheries and Aquatic Sciences, 25(7), TRJFAS26650. http://doi.org/10.4194/TRJFAS26650
Torrecillas, S., Montero, D., & Izquierdo, M. (2014). Improved health and growth of fish fed mannan oligosaccharides: potential mode of action. Fish & shellfish immunology, 36(2), 525–544. https://doi.org/10.1016/j.fsi.2013.12.029
Veenstra, L. D., Jannink, J.-L. & Sorrells, M. E. (2017). Wheat Fructans: A Potential Breeding Target for Nutritionally Improved, Climate-Resilient Varieties. Crop Science, 57, 1-17. https://doi.org/10.2135/CROPSCI2016.11.0955
Wei P, Zhao F, Wang Z, Wang Q, Chai X, Hou G, Meng Q. (2022). Sesame (Sesamum indicum L.): A Comprehensive Review of Nutritional Value, Phytochemical Composition, Health Benefits, Development of Food, and Industrial Applications. Nutrients. 14(19):4079. doi: 10.3390/nu14194079
Zarantoniello, M., Chemello, G., Ratti, S., Pulido-Rodriguez, L. F., Andiso, E., Freddi, L., Salinetti, P., Nartea, A., Bruni, L., Parisi, G., Riolo, P., & Olivotto, I. (2023). Growth and Welfare Status of Giant Freshwater Prawn (Macrobrachium rosenbergii) Post-Larvae Reared in Aquaponic System and Fed Diets including Enriched Black Soldier Fly (Hermetia illucens) Prepupae Meal. Animals, 13(715), 1-19. https://doi.org/10.3390/ani13040715
Zhang, Q., Tan, B., Mai, K., Zhang, W., Ma, H., Ai, Q., Wang, X., & Liu, Z. (2011). Dietary Administration of Bacillus (B. licheniformis and B. subtilis) and Isomaltooligosaccharide Influences The Intestinal Microflora, Immunological Parameters and Resistance Against Vibrio alginolyticus in Shrimp, Penaeus japonicus (Decapoda: Penaeidae). Aquaculture Research, 42, 943–952. 10.1111/j.1365-2109.2010.02677.x
Zhao, C., Zhu, J., Hu, J., Dong, X., Sun, L., Zhang, X., & Miao, S. (2019). Effects of Dietary Bacillus pumilus on Growth Performance, Innate Immunity and Digestive Enzymes of Giant Freshwater Prawns (Macrobrachium rosenbergii). Aquaculture Nutrition, 1–9. https://doi.org/10.1111/anu.12894
Zhou, H., Gai, C., Ye, G., An, J., Liu, K., Xu, L., Cao, H. (2019). Aeromonas hydrophila, an Emerging Causative Agent of Freshwater-Farmed Whiteleg Shrimp Litopenaeus vannamei. Microorganisms, 7(10), 1–20. https://doi.org/10.3390/microorganisms7100450
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